Product Description

ATP2B2 Polyclonal Antibody

Catalogue Numbers: BS60204-50, BS60204-100

Sizes: 50µl, 100µl

Alternative Name: Plasma membrane calcium-transporting ATPase 2; PMCA2; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2; PMCA2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q01814

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Plasma membrane-type Ca2+-ATPases (PMCAs) mediate the export of bivalent calcium ions from eukaryotic cells. As members of the P class of ion-motive ATPases, PMCAs are a functionally diverse group of proteins that are derived from alternatively spliced transcripts originating from four distinct genes, PMCA1, 2, 3, and 4. The expression of different PMCA isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, and with respect to the physiological needs of specific cell and tissue types. Spatial and temporal rates of resting intracellular Ca2+ concentrations and Ca2+ signaling in eukaryotic cells are dependent on the array of PMCA isoforms that are expressed in concert with the rate of Ca2+ export. The human PMCA2 gene is located on chromosome 3, and antibodies directed against PMCA2 detect three proteins in brain and heart. Homozygous null mutations in the mouse gene result in deafwaddler mice, which are characterized by having a hesitant, wobbly gait, displaying head bobbing, and are deaf.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATP2B2 polyclonal antibody detects endogenous levels of ATP2B2 protein.

Bioworld Molecular Weight: ~ 136 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ATP2B2 pAb at 1:500 dilution
Lane 1: A2780 whole cell lysate(40ug)
Lane 2: L02 whole cell lysate(40ug)
Lane 3: AML-12 whole cell lysate(40ug)
Lane 4: C6 whole cell lysate(40ug)

Immunogen: A synthetic peptide corresponding to residues in Human ATP2B2.

Conjugate: Unconjugated

Modification: Unmodified