ATP5A (K239) Polyclonal Antibody-BS3814

ATP5A (K239) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3814-50, BS3814-100

Sizes: 50µl, 100µl

Swiss-Prot: P25705

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes, F1, the hydrophilic catalytic core, and F0, the membrane-embedded protein channel. F1 consists of three α chains and three β chains, which are weakly homologous, as well as one γ chain, one δ chain and one ε chain. F0 consists of three subunits, a, b and c. The α chain of F1 is a regulatory subunit that contains 509 amino acids. Mitochondrial ATPase α chain (ATP5A) localizes to the mitochondria and catalyzes ATP synthesis.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ATP5A (K239) polyclonal antibody detects endogenous levels of ATP5A protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ATP5A (K239) pAb at 1:1000 dilution
Lane 1: AML-12 whole cell lysate (40ug)
Lane 2: PC12 whole cell lysate (40ug)
Lane 3: HepG2 whole cell lysate (40ug)
Lane 4: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ATP5A (K239) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 60 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: ATP synthase subunit alpha, mitochondrial; ATP5A1; ATP5A; ATP5AL2; ATPM

Immunogen: Synthetic peptide, corresponding to amino acids 210-260 of Human ATP5A.

Conjugate: Unconjugated

Modification: Unmodified