Product Description

ATP5A1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90109-50, BS90109-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P25705(Human) Q03265(Mouse) P15999(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three α chains and three β chains, which are weakly homologous, as well as one γ chain, one δ chain and one e chain. F0 consists of three subunits: a, b and c. The α chain of F1 is a regulatory subunit that contains 509 amino acids. Mitochondrial ATPase α chain (ATP5A) localizes to the mitochondria and catalyzes ATP synthesis.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ATP5A1 polyclonal antibody detects endogenous levels of ATP5A1 protein.

Bioworld Molecular Weight: 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ATP5A1 on different cells lysates using anti-ATP5A1 antibody at 1/500 dilution.
Positive control:
Line 1: Hela
Line 2: MCF-7, ICC staining ATP5A1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ATP5A1, ATP synthase alpha chain, mitochondrial, ATP synthase subunit alpha, ATP synthase subunit alpha mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2, ATP sythase (F1 ATPase) alpha subunit, ATP5A, Atp5a1, ATP5AL2, ATPA, ATPM, Epididymis secretory sperm binding protein Li 123m, hATP1, HEL-S-123m, MC5DN4, mitochondrial, Mitochondrial ATP synthetase, Mitochondrial ATP synthetase oligomycin resistant, Modifier of Min 2 mouse homolog, Modifier of Min 2, mouse, homolog of, MOM2, OMR, ORM, OTTHUMP00000163475,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified