ATP6V0C Polyclonal Antibody Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Sizes: 50µl, 100µl Catalogue Numbers: BS80014-50, BS80014-100 Swiss-Prot: P27449 Host: Rabbit ATP6V0C Polyclonal Antibody-BS80014 Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Reactivity: Human, Mouse, Rat Applications: WB, IF/ICC All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:100 Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c' , c" , and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Specificity: Unmodification Bioworld Molecular Weight: 16KDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of variousLysates, using ATP6V0C antibody at 1:1370 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s., Immunofluorescence analysis of MCF7 cells using ATP6V0C Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining. Alternative Name: ATP6V0C; ATP6C; ATP6L; ATPL; VATL; VPPC; Vma3 Immunogen: A synthetic peptide of human ATP6V0C(NP_001185498.1). Modification: Unmodified Conjugate: Unconjugated