ATP6V0D1 Monoclonal Antibody-MB10594

ATP6V0D1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10594-50, MB10594-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P61421

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis . May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium . In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 40 kDa; Observed MW: 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ATP6V0D1 in K562, rat Brain, C6, 3T3, Hela lysates using ATP6V0D1 antibody., Immunocytochemistry analysis of ATP6V0D1 in Hela using ATP6V0D1 antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human colon cancer using ATP6V0D1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: P39; VATX; VMA6; ATP6D; ATP6DV; VPATPD

Immunogen: Recombinant protein of human ATP6V0D1

Conjugate: Unconjugated

Modification: Unmodified