ATP6V1H Polyclonal Antibody-BS62511

ATP6V1H Polyclonal Antibody

Catalogue Numbers: BS62511-50, BS62511-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UI12

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis and an integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c’ and c”) make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The H subunit of V-ATPase, also designated SDF is comprised of two polypeptides derived from the same gene. This regulatory subunit plays a critical role in the functional coupling of ATP hydrolysis activity to proton transport in the V-ATPase pump.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATP6V1H polyclonal antibody detects endogenous levels of ATP6V1H protein.

Extra Notes: Western blot (WB) analysis of ATP6V1H polyclonal antibody at 1:500 dilution
LaneA:H9C2 whole cell lysate
LaneB:MEF whole cell lysate
LaneC:A549 whole cell lysate
LaneD:HepG2 whole cell lysate

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: V-type proton ATPase subunit H; V-ATPase subunit H; Nef-binding protein 1; NBP1; Protein VMA13 homolog; V-ATPase 50/57 kDa subunits; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD; ATP6V1H; CGI-11

Immunogen: Synthetic peptide, corresponding to Human ATP6V1H.

Conjugate: Unconjugated

Modification: Unmodified