Product Description

Aurora A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90112-50, BS90112-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14965(Human) P97477(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100
ICC:1:100-1:500

Background: Activation of the oncogenic protein kinase Aurora A regulates meiotic and mitotic cell cycles in eukaryotic cells. Specifically, Aurora A plays a key role in G2/M progression. Activation occurs via autophosphorylation, and while 14 sites are subject to this, only the threonine residue at position 295 is required for activity. Though autophosphorylation mediates activation, a number of other proteins influence activation, including the spindle assembly factor TPX2 and p53

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Aurora A polyclonal antibody detects endogenous levels of Aurora A protein.

Bioworld Molecular Weight: 46 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Aurora A in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Aurora A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ARK-1, Aurora-related kinase 1, hARK1, AURKA, AIK, ARK-1, ARK1, AURA, Aurka, Aurora 2, Aurora A, Aurora kinase A, Aurora-related kinase 1, Aurora/IPL1 like kinase, Aurora/IPL1-related kinase 1, AURORA2, Breast tumor-amplified kinase, BTAK, hARK1, IAK, IPL1 related kinase, MGC34538, OTTHUMP00000031340, OTTHUMP00000031341, OTTHUMP00000031342, OTTHUMP00000031343, OTTHUMP00000031344, OTTHUMP00000031345, OTTHUMP00000166071, OTTHUMP00000166072, PPP1R47, Protein phosphatase 1, regulatory subunit 47, Serine/threonine kinase 15, Serine/threonine kinase 6, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, STK15, STK6, STK6, STK7,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified