Product Description

Aven (P329) Polyclonal Antibody

Catalogue Numbers: BS2216-50, BS2216-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NQS1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: The Bcl-2 family regulates apoptosis in response to a wide range of stimuli through control of mitochondrial cytochrome c release and caspase activation. Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3. A large number of proteins have been found to interact with Bcl-2 and other family members that have been shown to help regulate apoptosis. Aven was identified in a yeast two-hybrid screen as a bcl-xL interacting protein. It also interacts with other anti-apoptotic family members, including Bcl-2, but fails to interact with pro-apopotic proteins Bax and Bak. Aven inhibits apoptosis and enhances anti-apopotic activity of Bcl-xL. It interferes with association with Apaf-1 and activation of caspase-9. Aven overexpression is associated with poor prognosis in acute lymphoblastic leukemia.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Aven pAb detects endogenous levels of Aven protein.

Extra Notes: Western blot (WB) analysis of Aven (P329) pAb at 1:1000 dilution
Lane1:HCT116 whole cell lysate(40ug)
Lane2:L02 whole cell lysate(40ug)
Lane3:The Testis tissue lysate of Mouse(40ug)
Lane4:The Heart tissue lysate of Rat(40ug)

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Myosin-15; Myosin heavy chain 15; MYH15; KIAA1000

Immunogen: Synthetic peptide, corresponding Human MYH15.

Conjugate: Unconjugated

Modification: Unmodified