Product Description

B-Raf Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9098-50, MB9098-100

Product: Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P15056(Human) P28028(Mouse)

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC

All Applications: WB:1:500-1:2,000
ICC:1:100-1:500
IHC:1:100-1:500

Background: Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: B-Raf monoclonal antibody detects endogenous levels of B-Raf protein.

Bioworld Molecular Weight: 84 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of B Raf on SiHa cell lysate using anti-B Raf antibody at 1/1,000 dilution., ICC staining B Raf (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: BRAF, BRAF1, RAFB1, FLJ95109, 94 kDa B raf protein, B raf 1, B Raf proto oncogene serine threonine protein kinase, B Raf proto oncogene, serine/threonine kinase, B RAF1, B-Raf proto-oncogene serine/threonine-protein kinase (p94), BRAF 1, BRAF, BRAF, BRAF1, cRmil, MGC126806, MGC138284, Murine sarcoma viral (v-raf) oncogene homolog B1, Murine sarcoma viral v raf oncogene homolog B1, NS7, Oncogen BRAF, oncogene BRAF1, p94, Proto-oncogene B-Raf, Proto-oncogene c-Rmil, RAFB 1, RAFB1, RMIL, Serine/threonine-protein kinase B-raf, v raf murine sarcoma viral oncogene homolog B, v raf murine sarcoma viral oncogene homolog B1, v-Raf murine sarcoma viral oncogene homolog B1,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified