B-Raf (Phospho-T401) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94049-50, BS94049-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P15056(Human) P28028(Mouse) EntrezGene:114486(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IP,
All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
Background: Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: B-Raf (Phospho-T401) polyclonal antibody detects endogenous levels of B-Raf protein only when phosphorylated at T401.
Bioworld Molecular Weight: 84 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Phospho-B Raf(T401) on different lysates using anti-Phospho-B Raf(T401) antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse testis
Lane 2: PC-12, ICC staining Phospho-B Raf(T401) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: BRAF, FLJ95109, 94 kDa B raf protein, B raf 1, B Raf proto oncogene serine threonine protein kinase, B Raf proto oncogene, serine/threonine kinase, B RAF1, B-Raf proto-oncogene serine/threonine-protein kinase (p94), BRAF 1, BRAF, BRAF, BRAF1, cRmil, MGC126806, MGC138284, Murine sarcoma viral (v-raf) oncogene homolog B1, Murine sarcoma viral v raf oncogene homolog B1, NS7, Oncogen BRAF, oncogene BRAF1, p94, Proto-oncogene B-Raf, Proto-oncogene c-Rmil, RAFB 1, RAFB1, RMIL, Serine/threonine-protein kinase B-raf, v raf murine sarcoma viral oncogene homolog B, v raf murine sarcoma viral oncogene homolog B1, v-Raf murine sarcoma viral oncogene homolog B1,
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr401 of human B Raf.
Conjugate: Unconjugated
Modification: Phosphorylation