Product Description

B2M Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90115-50, BS90115-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P61769(Human) P01887(Mouse) P07151(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IP,

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200

Background: Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an a heavy chain that contains three subdomains (α1, α2, α3), and a non-covalent associating light chain, known as β-2-Microglobulin. β-2-Microglobulin associates with the a3 subdomain of the a heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The α1 and α2 domains of the a heavy chain form the peptide antigen-binding cleft. Mice that lack β-2-Microglobulin protein show a normal distribution of T cells, yet have no mature CD4-8+ T cells and are defective in CD4-8+ T cell-mediated cytotoxicity. Interferon-γ can stimulate production of β-2-Microglobulin transcripts. The human β-2-Microglobulin gene maps to chromosome 15q21-q22.2 and encodes a 119 amino acid protein. Mutations in the β-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: B2M polyclonal antibody detects endogenous levels of B2M protein.

Bioworld Molecular Weight: 14 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of beta 2 Microglobulin on different lysates using anti-beta 2 Microglobulin antibody at 1/1,000 dilution.
Positive control:
Lane 1: Raji
Lane 2: U937, ICC staining beta 2 Microglobulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: B2M, B2M, B2MG, Beta 2 microglobin, Beta 2 microglobulin, Beta 2 microglobulin precursor, Beta chain of mhc class 1 proteins, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, CDABP0092, Hdcma22p,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified