B7-1/CD80 Polyclonal Antibody Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Sizes: 50µl, 100µl Catalogue Numbers: BS65576-50, BS65576-100 Swiss-Prot: Q00609 Host: Rabbit B7-1/CD80 Polyclonal Antibody-BS65576 Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Reactivity: Mouse, Applications: WB, All Applications: WB=1:500-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. Background: CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. Purification and Purity: affinity purified by Protein A Specificity: B7-1/CD80 polyclonal Antibody detects endogenous levels of B7-1/CD80 protein. Bioworld Molecular Weight: ~60kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Anti-B7-1/CD80 at 1/1000 dilution, Anti-B7-1/CD80 at 1/1000 dilution Alternative Name: Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_MOUSE; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80. Immunogen: KLH conjugated synthetic peptide derived from mouse B7-1/CD80:1-100/306 Modification: Unmodified Conjugate: Unconjugated