Product Description

BAF155 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66543-50, MB66543-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: ATP-dependent chromatin remodeling complexes play an essential role in the regμlation of nuclear processes such as transcription and DNA replication and repair. The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecμle of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regμlatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin. The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regμlation of gene activation, cell growth, cell cycle, and differentiation processes. Asymmetric dimethyation of SMARCC1/BAF155 by CARM1 was found to be associated with genes upregμlated by c-Myc and breast cancer progression. Furthermore, asymmetric dimethylated SMARCC1/BAF155 was found to be associated with chromatin independent of SWI/SNF ATPases Brg1 and BRM, suggesting a subcomplex capable of affecting chromatin state. Indeed, unmethylated SMARCC1/BAF155 seems to play a role in development as it more closely associates with Brg1 during development, which reduces pluripotency.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB

Alternate Names: BAF155, SWI/SNF complex subunit SMARCC1, BRG1-associated factor 155, BAF155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regμlator of chromatin subfamily C member 1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human SMARCC1 protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of BAF155 protein.

Molecular Weight: ~ 155 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q92922

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only