Product Description

BAF155 Monoclonal Antibody

Catalogue Numbers: MB66543-50, MB66543-100

Sizes: 50µl, 100µl

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q92922

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair. The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin. The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes.
Asymmetric dimethyation of SMARCC1/BAF155 by CARM1 was found to be associated with genes upregulated by c-Myc and breast cancer progression. Furthermore, asymmetric dimethylated SMARCC1/BAF155 was found to be associated with chromatin independent of SWI/SNF ATPases Brg1 and BRM, suggesting a subcomplex capable of affecting chromatin state. Indeed, unmethylated SMARCC1/BAF155 seems to play a role in development as it more closely associates with Brg1 during development, which reduces pluripotency.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of BAF155 protein.

Extra Notes: Western blot analysis of BAF155 expression in K562 (A), Jurkat (B), Hela (C) whole cell lysates.

Bioworld Molecular Weight: ~ 155 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: BAF155; SWI/SNF complex subunit SMARCC1; BRG1-associated factor 155; BAF155; SWI/SNF complex 155 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1

Immunogen: Purified recombinant human SMARCC1 protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified