Product Description

BAF53 (W237) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1910-50, BS1910-100

Sizes: 50µl, 100µl

Swiss-Prot: O96019

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The SWI/SNF complex regulates gene expression via ATP-dependent chromatin remodeling. Brm (SNF2-a), Brg-1 (SNF2-b), Ini1 (integrase interactor 1, SNF5), BAF53 (ARPnβ), BAF57, BAF155 (SRG3), and BAF170 make up the functional core. BAF53 homologues from yeast to humans contain a conserved N-terminal motif, which contains residues at serine 2 and tyrosine 6, which play important roles in BAF53 activity. The BAF53 protein shuttles between the nucleus and cytoplasm. BAF53 also forms a complex with TIP49 and TIP48, which mediates c-Myc oncogenic activity.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: BAF53 (W237) polyclonal antibody detects endogenous levels of BAF53 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of BAF53 (W237) pAb at 1:500 dilution
Lane 1: PC3 whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: The Uterus tissue lysate of Rat (40ug)
Lane 4: The Uterus tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of BAF53 (W237) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 47 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Actin-like protein 6A; 53 kDa BRG1-associated factor A; Actin-related protein Baf53a; ArpNbeta; BRG1-associated factor 53A; BAF53A; INO80 complex subunit K; ACTL6A; BAF53; BAF53A; INO80K

Immunogen: Synthetic peptide, corresponding to amino acids 211-260 of Human BAF53.

Conjugate: Unconjugated

Modification: Unmodified