Product Description

Bag-4 (V442) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS9179-50, BS9179-100

Sizes: 50µl, 100µl

Swiss-Prot: O95429

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The cytokine TNF (tumor necrosis factor) signals through the TNF-R1 receptor to activate various cellular pathways, including apoptosis and NFkB activation. TNF binding induces receptor aggregation, resulting in the recruitment of TRADD, FADD, TRAF2 and RIP to the intracellular “death” domain of the receptor complex, which in turn activates signaling pathways including apoptosis and NFkB activation. SODD, for silencer of death domains, was found to be associated with the intracellular “death” domain of TNF-R1 in the absence of TNF stimulation. TNF treatment results in the release of SODD from TNF-R1, allowing the recruitment of TRADD and TRAF2 to the receptor complex. Thus, SODD may play a role in preventing spontaneous signaling by death-domain receptors, in the absence of ligand.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Bag-4 (V442) polyclonal antibody detects endogenous levels of Bag-4 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Bag-4 (V442) pAb at 1:500 dilution
Lane 1: A375 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: Hela whole cell lysate (40ug)
Lane 4: 3T3-L1 whole cell lysate (40ug)
Lane 5: PC12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Bag-4 (V442) pAb in paraffin-embedded human breast cancer tissue.

Bioworld Molecular Weight: ~ 49 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: BAG 4; BAG family molecular chaperone regulator 4; BAG-4; BAG4; BAG4; Bcl 2 associated athanogene 4; Bcl-2-associated athanogene 4; BCL2 associated athanogene 4; Silencer of death domains; SODD;

Immunogen: Synthetic peptide, corresponding to amino acids 411-460 of Human Bag-4.

Conjugate: Unconjugated

Modification: Unmodified