BAG1 Polyclonal Antibody
Catalogue Numbers: BS60848-50, BS60848-100
Sizes: 50µl, 100µl
Alternative Name: BAG family molecular chaperone regulator 1; BAG-1; Bcl-2-associated athanogene 1; BAG1; HAP
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q99933
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:202
Background: The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis, while other members of the Bcl-2 family, such as Bax and Bak, enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis. Dimerization of another member of this family, Bad, with Bcl-xL, results in displacement of Bax from Bcl-xL/Bax complexes and restoration of Bax-mediated apoptosis. A Bcl-2-binding protein, designated Bag-1, lacks significant homology with Bcl-2 or with other Bcl-2-related proteins. Bag-1 appears to function to enhance Bcl-2 protection from cell death, suggesting that Bag-1 represents a new type of anti-cell death gene. This also suggests that certain routes of apoptosis induction, previously ascribed to Bcl-2-independent pathways, may instead reflect a requirement for a combination of Bcl-2 and Bag-1.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: BAG1 polyclonal antibody detects endogenous levels of BAG1 protein.
Bioworld Molecular Weight: ~ 39 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of BAG1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human BAG1.
Conjugate: Unconjugated
Modification: Unmodified