Product Description

Bak Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10609-50, MB10609-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q16611

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: Bak is a proapoptotic member of the Bcl-2 family. This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway. Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 23 kDa; Observed MW: 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Bak in K562, Hela lysates using Bak antibody., Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Bak antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: BAK1; BAK; BCL2L7; CDN1; Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7

Immunogen: A synthetic peptide of human Bak

Conjugate: Unconjugated

Modification: Unmodified