Product Description

Bak Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16594-50, BZ16594-100

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Swiss-Prot: Q16611

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-P, ICC/IF, IP, , FC

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100

Background: Bak is a proapoptotic member of the Bcl-2 family. This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway. Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 23 kDa; Observed MW: 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Bak in HeLa lysates using Bak antibody., Immunohistochemistry analysis of paraffin-embedded mouse liver using Bak antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunofluorescence analysis of Bak in Hela using Bak antibody.

Alternative Name: BAK1; BAK; BCL2L7; CDN1; Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7

Immunogen: A synthesized peptide derived from human Bak

Conjugate: Unconjugated

Modification: Unmodified