Product Description

Bak Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9917M-50, BS9917M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16611

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:2000
IHC/ICC/IF: 1:50-1:200
FC: 1:50-1:100

Background: Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Bak and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Bak Rabbit mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: The skeletal muscle lysate of Human
Lane 3: AGS whole cell lysate, Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Bak antibody. Counter stained with hematoxylin., ICC staining Bak in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK; BCL2L7; CDN1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified