BAP31 Monoclonal Antibody-MB67011

BAP31 Monoclonal Antibody

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB67011-50, MB67011-100

Sizes: 50µl, 100µl

Swiss-Prot: P51572

Host: Mouse

Reactivity: Human

Applications: WB, IHC

Background: BAP31 (B-cell receptor-association protein 31) is a transmembrane protein associated with the endplasmic reticulum (ER) and ER-Golgi intermediates and has been implicated in protein trafficking and apoptosis . During apoptosis Bap31 is cleaved by caspase-8 at two carboxy-terminal sites which can then direct apoptotic signals between the ER and mitochondria . Association of BAP31 with the anti-apoptotic proteins Bcl-2 or Bcl-xL could function to regulate this ER-mitochondrial pathway . Several studies have shown that BAP31 can control the trafficking of select proteins between the ER and Golgi apparatus and can affect the transport of proteins to the cell surface .

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of BAP31 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of BAP31 expression in A431 (A), NCCIT (B) whole cell lysates., Immunohistochemical analysis of BAP31 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 28 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: BAP31; DXS1357E; B-cell receptor-associated protein 31; BCR-associated protein 31; Bap31; 6C6-AG tumor-associated antigen; Protein CDM; p28

Immunogen: Recombinant fusion protein of human BAP31. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified