Product Description

Bax Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9013-50, MB9013-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q07812

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, ELISA IHC, IF ICC

All Applications: WB: 1:1000-1:1500
ELISA: 1:5000-1:10000
IHC: 1:100-1:500
IF: 1:200-1:500
ICC: 1:100

Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Bax Mouse monoclonal antibody detects endogenous levels of Bax protein.

Bioworld Molecular Weight: ~ 21 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Primary: Anti-Bax at 1/1000 dilution, Primary: Anti-Bax at 1/1000 dilution, Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4°C., Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Alternative Name: Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX; BCL2L4

Immunogen: KLH conjugated synthetic peptide derived from human Bax: 31-130/192

Conjugate: Unconjugated

Modification: Unmodified