Product Description

BAX Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90120-50, BS90120-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q07812(Human) Q07813(Mouse) Q63690(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC

All Applications: WB:1:500-1:1,000
ICC:1:200
IHC:1:200

Background: BAX is a member of the Bcl-2 gene family. Apoptosis regulator BAX promotes apoptosis by binding to and antagonizing the Bcl-2 protein. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BAX.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: BAX polyclonal antibody detects endogenous levels of BAX protein.

Bioworld Molecular Weight: 21 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Bax on different lysates using anti-Bax antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Hela
Lane 3: Mouse embryonic stem cell
Lane 4: PC12
Lane 5: F9, ICC staining Bax in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Bcl2-L-4, Apoptosis regulator BAX, BAX, Bax-protein, BAX, BAXA, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, Bcl2-L-4, BCL2L4, membrane isoform alpha,

Immunogen: This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal BAX.

Conjugate: Unconjugated

Modification: Unmodified