Product Description

Bax Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90121-50, BS90121-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q07812(Human) Q07813(Mouse) Q63690(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP, , FC

All Applications: WB:1:1,000-1:5,000
IHC:1:50-1:200
FC:1:50-1:100

Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax (Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activity of Bcl-2.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Bax polyclonal antibody detects endogenous levels of Bax protein.

Bioworld Molecular Weight: 21,15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Bax on different lysates using anti-Bax antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7
Lane 3: Human liver, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bax antibody. Counter stained with hematoxylin.

Alternative Name: Bcl2-L-4, Apoptosis regulator BAX, BAX, Bax-protein, BAX, BAXA, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, Bcl2-L-4, BCL2L4, membrane isoform alpha,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified