Product Description

BCAS2 Polyclonal Antibody

Catalogue Numbers: BS5626-50, BS5626-100

Sizes: 50µl, 100µl

Alternative Name: Pre-mRNA-splicing factor SPF27; Breast carcinoma-amplified sequence 2; DNA amplified in mammary carcinoma 1 protein; Spliceosome-associated protein SPF 27; DAM1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75934

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC IP

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200
IP: 1:50~1:200

Background: BCAS2 (breast carcinoma amplified sequence 2), also designated DAM1 (DNA amplified in mammary carcinoma 1 protein) or spliceosome-associated SPF 27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. BCAS2 is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligandindependent manner) with TRβ (thyroid hormone receptor β), ERα (estrogen receptor α), ERβ, PR (progesterone receptor), and PPARγ (Peroxisome proliferator-activated receptor γ). BCAS2 functions as an ER coactivator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: BCAS2 polyclonal antibody detects endogenous levels of BCAS2 protein.

Bioworld Molecular Weight: ~ 26 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of BCAS2 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: Mouse heart tissue lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 180-225 of Human BCAS2.

Conjugate: Unconjugated

Modification: Unmodified