Product Description

BCKDHA Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67347-50, BS67347-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascμlar and metabolic disorders. The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway. Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex. The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regμlation of BCKDH. Phosphorylation of BCKDH-E1α was shown to play a key role in regμlating the enzymatic activity of this complex. Phosphorylation of BCKDH-E1α at Ser293 inactivates BCKDH. A significant elevation in plasma BCAA levels was reported to correlate with increased phosphorylation of BCKDH-E1α at Ser293 and suppressed BCKDH activity in the liver of diabetic mice.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF/ICC

Alternate Names: 2-oxoisovalerate dehydrogenase subunit alpha mitochondrial, Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, BCKDE1A, BCKDH E1-alpha

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fμll length protein of human BCKDHA

Dilution: WB (1/500 - 1/2000), IF/ICC (1/50 - 1/200)

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of BCKDHA protein.

Molecular Weight: ~50 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P12694

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only