Product Description

Bcl-2 (P65) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0368-50, AP0368-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Bcl-2 exerts a survival function in response to a wide range of apoptotic stimμli through inhibition of mitochondrial cytochrome c release. It has been implicated in modμlating mitochondrial calcium homeostasis and proton flux. Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87. It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events. Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes. Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF

Alternate Names: Bcl-2 homologous antagonist/killer, Apoptosis regμlator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to Human Bcl-2.

Dilution: WB: 1:1000~1:2000
IF: 1:100~1:500

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Bcl-2 (P65) polyclonal antibody detects endogenous levels of Bcl-2 protein.

Molecular Weight: ~ 30 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P10415

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only