Bcl-2 (P65) Polyclonal Antibody-AP0368

Bcl-2 (P65) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP0368-50, AP0368-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q16611

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB: 1:1000~1:2000

Background: Bak is a proapoptotic member of the Bcl-2 family. This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway. Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Bcl-2 (P65) polyclonal antibody detects endogenous levels of Bcl-2 protein.

Bioworld Molecular Weight: ~ 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Bcl-2 (P65) polyclonal antibody at 1:1000 dilution
Lane 1: The Kidney tissue lysate of Rat (30ug)
Lane 2: The Heart tissue lysate of Mouse (30ug), Western blot (WB) analysis of Bcl-2 (P65) pAb at 1:1000 dilution
Lane 1: The Kidney tissue lysate of Rat (30ug)
Lane 2: A2780 whole cell lysate (30ug)
Lane 3: A549 whole cell lysate (30ug)

Alternative Name: Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK; BCL2L7; CDN1

Immunogen: Synthetic peptide, corresponding to Human Bcl-2.

Conjugate: Unconjugated

Modification: Unmodified