Product Description

Bcl-2 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65626-50, BS65626-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14, 18)-bearing follicμlar B cell lymphomas(1, 2).Bcl-2 blocks cell death following a variety of stimμli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3, 5).Bcl-2 appears to function in several subcellμlar locations yet lacks any known motifs that woμld confer insight into its mechanism of action (6, 7).A more recently identified protein, designated Bax p21(i.e., Bcl-associated X protein ), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF/ICC

Alternate Names: Apoptosis regμlator Bcl-2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCL-2.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF/ICC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Bcl-2 Polyclonal Antibody detects endogenous levels of Bcl-2 protein.

Molecular Weight: 28 kD

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P49950

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only