Bcl-2 Polyclonal Antibody-BS65626

Bcl-2 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65626-50, BS65626-100

Swiss-Prot: P49950

Host: Rabbit

Bcl-2 Polyclonal Antibody-BS65626

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Mouse, Rat, (predicted: Human, )

Applications: WB, IHC-P

All Applications: WB=1:500-2000 

Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14; 18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).

Purification and Purity: affinity purified by Protein A

Specificity: Bcl-2 Polyclonal Antibody detects endogenous levels of Bcl-2 protein.

Bioworld Molecular Weight: 26 kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample:
Lane 1: Spleen (Rat) Lysate at 40 ug
Lane 2: Bone (Rat) Lysate at 40 ug
Primary:
Anti- Bcl-2 at 1/1000 dilution, Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Lane 3: Bone (Mouse) Lysate at 40 ug
Lane 4: Thyroid gland (Mouse) Lysate at 40 ug
Lane 5: Spleen (Rat) Lysate at 40 ug
Primary:
Anti- Bcl-2 at 1/1000 dilution

Alternative Name: Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.

Immunogen: KLH conjugated synthetic peptide derived from human Bcl-2

Modification: Unmodified

Conjugate: Unconjugated