Product Description

Bcl-2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65629-50, BS65629-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P49950

Host: Rabbit

Reactivity: Human, Mouse, Rat,

Applications: WB

All Applications: WB=1:500-2000

Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Bcl-2 Polyclonal Antibody detects endogenous levels of Bcl-2 protein.

Bioworld Molecular Weight: 26 kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Lane 3: Lymph node (Rat) Lysate at 40 ug
Lane 4: MCF-7 (Human) Cell Lysate at 30 ug
Lane 5: SiHa (Human) Cell Lysate at 30 ug
Lane 6: Jurkat (Human) Cell Lysate at 30 ug
Lane 7: A431 (Human) Cell Lysate at 30 ug
Lane 8: Bone (Rat) Lysate at 40 ug
Lane 9: Bone (Mouse) Lysate at 40 ug
Lane 10: A549 (Human) Cell Lysate at 30 ug
Lane 11: SH-SY5Y (Human) Cell Lysate at 30 ug
Primary: Anti-Bcl-2 at 1/1000 dilution

Alternative Name: Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.

Immunogen: KLH conjugated synthetic peptide derived from human Bcl-2

Conjugate: Unconjugated

Modification: Unmodified