Bcl-2 Polyclonal Antibody-BS90127

Bcl-2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90127-50, BS90127-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10415(Human) P10417(Mouse) P49950(Rat)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:500
FC:1:50-1:100

Background: Apoptosis is defined as a set of cascades which, when initiated, programs the cell to undergo lethal changes such as membrane blebbing, mitochondrial break down and DNA fragmentation. Bcl-2 is one among many key regulators of apoptosis, which are essential for proper development, tissue homeostasis, and protection against foreign pathogens. Human Bcl-2 is an anti-apoptotic, membrane-associated oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xl, Mcl-1, Bcl-w, and A1 or the death agonists Bax, Bak, Bik, Bad, and Bid. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phospho-rylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Mutations in the Bcl-2 gene can contribute to cancers where normal physiological cell death mechanisms are compromised by deregulation of the anti-apoptotic influence of Bcl-2.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Bcl-2 polyclonal antibody detects endogenous levels of Bcl-2 protein.

Bioworld Molecular Weight: 26/22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Bcl-2 on different lysates using anti-Bcl-2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat, ICC staining Bcl-2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: BCL2, Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl-2, Bcl2, BCL2, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified