Product Description

Bcl-7a (N62) Polyclonal Antibody

Catalogue Numbers: BS9274-50, BS9274-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q4VC05

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Bcl-7a (B-cell CLL/lymphoma 7 protein family member A) is a 210 amino acid protein that belongs to the Bcl-7 family. Chromosomal aberrations involving a disruption of the amino-terminus of Bcl-7a may be a cause of B-cell nonHodgkin lymphoma (BNHL). Bcl-7b and Bcl-7c proteins share 90% identity with the N-terminal 51 amino acids of Bcl-7a, suggesting that the three genes are members of an evolutionarily conserved gene family. Bcl-7 gene family may be involved in early development. The Bcl-7a protein interacts with a novel human Polycomb ring finger gene and exists as two alternatively spliced isoforms. Bcl-7a may play a role in GC reaction and/or B-cell lymphomagenesis. The Bcl-7a gene maps to human chromosome 12q24.31. Chromosome 12 encodes over 1,100 genes and comprises approximately 4.5% of the human genome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Bcl-7a (N62) polyclonal antibody detects endogenous levels of Bcl-7a protein.

Extra Notes: Western blot (WB) analysis of BCL7A pAb at 1:500 dilution
Lane1:The Fallopian tube tissue lysate of Rat(40ug)
Lane2:The Embryo tissue lysate of Mouse(40ug)
Lane3:PC12 whole cell lysate(40ug)
Lane4:CT-26 whole cell lysate (40ug)
Lane5:PC3 whole cell lysate(40ug)
Lane6:A2780 whole cell lysate(40ug)
Lane7:LOVO whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 26 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: B cell CLL/lymphoma 7; B cell CLL/lymphoma 7 protein family member A; B cell CLL/lymphoma 7A; B-cell CLL/lymphoma 7 protein family member A; BCL 7; BCL7; bcl7a; BCL7A

Immunogen: Synthetic peptide, corresponding to amino acids 30-84 of Human Bcl-7a.

Conjugate: Unconjugated

Modification: Unmodified