Product Description

BCL-x Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-30640-60, E-AB-30640-120, E-AB-30640-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: Bcl-x

Target Synonym: Apoptosis regulator Bcl X; Apoptosis regulator Bcl-X; Apoptosis regulator BclX; B cell lymphoma 2 like; B2CL1; Bcl 2 like 1 protein; Bcl X; Bcl xL; BCL XL/S; Bcl xS; Bcl-2-like protein 1; Bcl2 Like 1; Bcl2 related gene; Bcl2-L-1; BCL2L; Bcl2l1; BCLX; BclXL; BclXs; DKFZp781P2092; PPP1R52; Protein phosphatase 1 regulatory subunit 52

Research Areas: Cancer, Cell Biology, Metabolism, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC-p, IF, ELISA

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q07817

Background: Bcl x is a member of the Bcl 2 family of proteins, which function as regulators of apoptosis. It may provide neuroprotection against ischemic brian injury. Bcl x has two isoforms, Bcl xl (long) a 241 amino acid protein and Bcl xs (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl2 family. Isoform Bcl-X(L) anti-apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, cytochrome c, from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis.

Concentration: 1 mg/mL

Immunogen: Synthesized peptide derived from human Bcl-x around the non-phosphorylation site of Thr47.

Buffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000

Calculated MW: 26kDa

Observed MW: 30kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only