Product Description

BDNF Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS66135-50, BS66135-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P23560

Host: Rabbit

Reactivity: Human, Mouse, Rat,

Applications: WB, ELISA IHC IF

All Applications: WB: 1:500-1:2000
ELISA: 1:5000-1:10000
IHC: 1:100-1:500
IF: 1:100-1:500

Background: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: BDNF Polyclonal Antibody detects endogenous levels of BDNF protein.

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample: Brain(Rat)lysate at 30ug;
Primary: Anti-BDNF at 1:200, Tissue/cell: rabbit brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-BDNF Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C., Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-BDNF Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C.

Alternative Name: Abrineurin; Brain Derived Neurotrophic Factor; MGC34632; Neurotrophin; BDNF_HUMAN

Immunogen: KLH conjugated synthetic peptide derived from human BDNF: 81-180/247

Conjugate: Unconjugated

Modification: Unmodified