Product Description

Beclin 1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP6020-50, AP6020-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: BECN1 (beclin 1) is a coiled-coil protein that has been implicated as an inhibitor of tumorigenesis. BECN1, which associates with Bcl-2, plays a significant role in autophagy. Autophagy is the degradation of cellμlar proteins in the lysosomes, and when this pathway is suppressed, cell growth is deregμlated. Autophagy is controlled by the same signal transduction pathway that induces the phosphorylation of the ribosomal protein S6, and both are mediated via amino acids. BECN1 expression in various carcinoma cell lines, such as MCF7, is low, whereas it is ubiquitously expressed in normal breast tissue. In transfected MCF7 cells, BECN1 complements autophagocytosis and, subsequently, inhibits cellμlar proliferation. Additionally, BECN1 shares structural similarity to the yeast autophagy gene product, APG6, and was one of the first mammalian proteins discovered to mediate autophagy.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: APG6, ATG 6, ATG6, ATG6 autophagy related 6 homolog, Bcl-2-interacting protein beclin, Beclin 1 (coiled coil moesin like BCL2 interacting protein), Beclin 1 autophagy related, Beclin-1, Beclin1, BECN 1, BECN1, BECN1, Coiled coil myosin like BCL2 interacting protein, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197 antibod

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Recombinant fragment corresponding to a region within amino acids 141-451 of Human Beclin 1.

Dilution: WB: 1:2000~1:5000
IHC: 1:50~1:200
IF: 1:100~1:500

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Beclin 1 polyclonal antibody detects endogenous levels of Beclin 1 protein.

Molecular Weight: ~ 55 kDa

Pathway: Autophagy Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q14457

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only