Product Description

beta actin Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-40338-60, E-AB-40338-120, E-AB-40338-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: ACTBB

Target Synonym: ACTB; Actin beta; Actx; Actin cytoplasmic 1; b actin; A26C1A

Research Areas: Isotype, Tags and Cell markers, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q7ZVF9

Background: Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Concentration: 0.85mg/mL

Immunogen: Recombinant Zebrafish Actin, cytoplasmic 2 protein

Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3.

Purification Method: Antigen Affinity Purification

Dilution: WB 1:3000-1:30000 IHC 1:100-1:300

Calculated MW: 41 kDa

Observed MW: 41 kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only