Beta-tubulin Rabbit Monoclonal Antibody-MB66321

Beta-tubulin Rabbit Monoclonal Antibody

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Catalogue Numbers: MB66321-50, MB66321-100

Sizes: 50µl, 100µl

Swiss-Prot: P07437

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP

Background: The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins.

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of Beta-tubulin protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Beta-tubulin expression in rat brain (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates., Immunohistochemical analysis of Beta-tubulin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.119). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 55 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: TUBB5; Tubulin beta chain; Tubulin beta-5 chain

Immunogen: A synthetic peptide of human beta Tubulin

Conjugate: Unconjugated

Modification: Unmodified