Beta1-tubulin Monoclonal Antibody-MB67208

Beta1-tubulin Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB67208-50, MB67208-100

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q9H4B7

Host: Mouse

Reactivity: Human, Mouse

Applications: WB, IHC, IF/ICC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50)

Background: Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Beta1-tubulin protein.

Bioworld Molecular Weight: ~ 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Beta1-tubulin expression in CCRFCEM (A), Hela (B) whole cell lysates., Immunohistochemical analysis of Beta1-tubulin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of Beta1-tubulin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Alternative Name: Tubulin beta-1 chain

Immunogen: Recombinant fusion protein of human Beta1-tubulin. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified