BHMT Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9090-50, MB9090-100
Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q93088(Human) 329582(Mouse) O09171(Rat)
Host: Mouse
Reactivity: Human, Mouse, Rat
All Applications: WB:1:1,000-1:5,000
ICC:1:50
IHC:1:50-1:200
FC:1:50-1:100
Background: Betaine-homocysteine methyltransferase (BHMT) is a zinc-dependent cytosolic protein that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT is highly expressed in rat liver, and its expression is regulated by dietary methionine and choline. In humans, S-adenosylmethionine (SAM) down-regulates BHMT expression by inducing NFκB, which acts as a repressor for the BHMT gene. Lowered BHMT levels can lead to ER (endoplasmic reticulum) stress. Mutations in the gene encoding for BHMT may lead to hyperhomocysteinemia, a medical condition characterized by abnormally large amounts of homocysteine in the blood which may be a risk factor for cardiovascular and cerebrovascular diseases.
Purification and Purity: ProG affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: BHMT monoclonal antibody detects endogenous levels of BHMT protein.
Bioworld Molecular Weight: 45 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of BHMT on human liver tissue and human kidney tissue lysate using anti-BHMT antibody at 1/1,000 dilution., ICC staining BHMT (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: BHMT, Betaine homocysteine methyltransferase, Betaine homocysteine S methyltransferase 1, Betaine homocysteine S methyltransferase, Betainehomocysteine S-methyltransferase 1, BHMT, BHMT1, BHMT1, EC 2.1.1.5, Epididymis secretory sperm binding protein Li 61p, HEL S 61p,
Immunogen: Recombinant protein.
Conjugate: Unconjugated
Modification: Unmodified