Product Description

BIK (Phospho-S35) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64571-50, BS64571-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13323

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Bik/Nbk (Bcl-2-interacting killer/natural born killer) is a potent pro-apoptotic protein belonging to a group of Bcl-2 family members that includes Bad, Bid, Bim, Hrk, and Noxa, containing a BH3 domain but lacking other conserved domains, BH1 or BH2. Functionally, Bik is able to bind to and antagonize anti-apoptotic Bcl-2 family members including Bcl-2, Bcl-xL, and viral homologs E1B-19K and EBV-BHFR1. The BH3 domain of Bik is essential for its apoptotic activity and interaction with survival proteins. Phosphorylation of Bik is correlated with an increase in its pro-apoptotic activity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: BIK (Phospho-S35) polyclonal antibody detects endogenous levels of BIK protein only when phosphorylated at Ser35.

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of BIK (Phospho-S35) polyclonal antibody at 1:500 dilution
Lane A: The Embryo tissue lysate of Mouse
Lane B: The Ovary tissue lysate of Rat
Lane C: HEK293T starved with EGF whole cell lysate
Lane D: HEK293T whole cell lysate

Alternative Name: Bcl-2-interacting killer; Apoptosis inducer NBK; BIP1; BP4; BIK; NBK

Immunogen: Synthetic phosphopeptide derived from human BIK around the phosphorylation site of Serine 35.

Conjugate: Unconjugated

Modification: Phosphorylation