Product Description

Bim Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90137-50, BS90137-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43521(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC, IHC, IP,

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Bim polyclonal antibody detects endogenous levels of Bim protein.

Bioworld Molecular Weight: 22/16/13 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Bim on different lysates using anti-Bim antibody at 1/1,000 dilution.
Positive control:
Lane 1: Raji
Lane 2: Jurkat
Lane 3: A431, ICC staining Bim in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Bcl2-L-11, BCL2L11, BCL2 like 11, B2L11, BAM, Bcl 2 interacting protein Bim, Bcl 2 related ovarian death agonist, Bcl-2-like protein 11, BCL2 interacting mediator of cell death, BCL2 like 11 (apoptosis facilitator), BCL2 like protein 11, Bcl2-interacting mediator of cell death, Bcl2-L-11, Bcl2l11, BIM alpha6, BIM, BIM beta6, BIM beta7, BimEL, BimL, BOD,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified