Biotin Anti-Mouse F4/80 Antibody (CI:A3-1)
Sizes: 25μg, 100μg
Catalogue Numbers: Biotin-30096U-25, Biotin-30096U-100
Citations, Manuals and MSDS Available upon request.
Target: Mouse F4/80
Form: Liquid
Conjugation: Biotin
Host: Rat
Clonality: Monoclonal
Clone ID: CI:A3-1
Isotype: IgG2b, κ
Storage: PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background: F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
Synonyms: Gpf480| Adgre1| Adhesion G protein-coupled receptor E1| Cell surface glycoprotein F4/80| EGF-like module receptor 1| Emr1
Uniprot ID: Q61549
Reactivity: Mouse
Tested Application: FC
Recommended Dilution: Each lot of this antibody is quality control tested by Flow cytometryometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].
Gene ID: 13733
Category: Flow cytometryometry Antibodies
Research Use Only