Biotin Anti-Mouse F4/80 Antibody [CI:A3-1] - E-AB-F0995B

Biotin Anti-Mouse F4/80 Antibody [CI:A3-1]

Size: 100μg

Catalogue Number: E-AB-F0995B-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: F4/80

Target Synonym: Adhesion G protein-coupled receptor E1, Adgre1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, Adgre1, Emr1, Gpf480,

Conjugation: Biotin

Host: Rat

Species Reactivity: Mouse

Application: FCM

Usage: Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10^6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Isotype: Rat IgG2b, κ

Clone No.: CI:A3-1

UNIProt ID: Q61549

Background: F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Concentration: 0.5 mg/mL

Control Catalogue Number: E-AB-F09843B

Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

GeneID: 13733

Storage: This product can be stored at 2-8°C for 12 months. Do not freeze.

Shipping: Ice bag