Bmi1 Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50 µl, 100 µl
Catalogue Numbers: BS6015-50, BS6015-100
Swiss-Prot: P35226
Host: Rabbit
Bmi1 Polyclonal Antibody-BS6015
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 41kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of U-937 cells, using Bmi1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s., Immunofluorescence analysis of HeLa cells using Bmi1 antibody. Blue: DAPI for nuclear staining.
Alternative Name: BMI1; FLVI2/BMI1; PCGF4; RNF51; flvi-2/bmi-1; Bmi1
Immunogen: Recombinant fusion protein of human Bmi1(NP_005171.4).
Modification: Unmodified
Conjugate: Unconjugated