Product Description

BMP2K Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS60064-50, BS60064-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q9NSY1

Host: Rabbit

Reactivity: Human

Applications: WB, IP,

All Applications: WB: 1:1000~1:2000
IP 1:50 - 1:200

Background: The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. BMP2K (BMP2 inducible kinase), also known as BIKE, is a 1,161 amino acid nuclear protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase family. Thought to be involved in osteoblast differentiation, BMP2K catalyzes the ATP-dependent phosphorylation of bone morphogenic proteins (BMPs); proteins that are essential for proper cartilage and bone formation. Via its catalytic activity, BMP2K may play a role in signaling pathways that mediate bone growth and cellular differentiation. Three isoforms of BMP2K exist due to alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: BMP2K polyclonal antibody detects endogenous levels of BMP2K protein.

Bioworld Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of BMP2K polyclonal antibody at 1:1000 dilution
Lane 1: A549 whole cell lysate (30ug)
Lane 2: MCF-7 whole cell lysate (30ug), Immunoprecipitation of MCF-7 cell lysates using BMP2K pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using BMP2K pAb #BS60064.

Alternative Name: BMP-2-inducible protein kinase; BIKe; BMP2K; BIKE; HRIHFB2017

Immunogen: Synthetic peptide, corresponding to Human BMP2K.

Conjugate: Unconjugated

Modification: Unmodified