Product Description

BMX (Phospho-Y566) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64517-50, BS64517-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51813

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Background: ETK, also known as BMX, is a member of the Bruton's tyrosine kinase (Btk) family. It is expressed in a variety of hematopoietic, epithelial and endothelial cells. Etk, like other Btk family members, contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. It participates in multiple signal transduction pathways. Phosphorylation of Tyr566 by Src kinase is required for activation of Etk in vivo. In endothelial and epithelial cells, Etk is regulated by FAK through phosphorylation at Tyr40.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: BMX (Phospho-Y566) polyclonal antibody detects endogenous levels of BMX protein only when phosphorylated at Tyr566.

Bioworld Molecular Weight: ~ 78 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of BMX (Phospho-Y566) polyclonal antibody at 1:500 dilution
Lane A: HEK293T whole cell lysate
Lane B: Hela whole cell lysate
Lane C: The Kidney tissue lysate of Mouse

Alternative Name: Cytoplasmic tyrosine-protein kinase BMX; Bone marrow tyrosine kinase gene in chromosome X protein; Epithelial and endothelial tyrosine kinase; NTK38; BMX

Immunogen: Synthetic phosphopeptide derived from human BMX around the phosphorylation site of Tyrosine 566.

Conjugate: Unconjugated

Modification: Phosphorylation