BPGM Polyclonal Antibody-BS70972

BPGM Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS70972-50, BS70972-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07738

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: 2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 30kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using BPGM antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of HeLa cells using BPGM Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: BPGM; DPGM

Immunogen: Recombinant fusion protein of human BPGM(NP_001715.1).

Conjugate: Unconjugated

Modification: Unmodified