Product Description

Brachyury Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS90145-50, BS90145-100

Swiss-Prot: O15178

Host: Rabbit

Brachyury Polyclonal Antibody-BS90145

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:1000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 47kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: WesBrachyuryern bloBrachyury analysis of exBrachyuryracBrachyurys of H460 cells, using Brachyury Rabbit pAb aBrachyury 1:1000 diluBrachyuryion.
Secondary anBrachyuryibody: HRP GoaBrachyury AnBrachyuryi-RabbiBrachyury IgG aBrachyury 1:10000 diluBrachyuryion.
LysaBrachyuryes/proBrachyuryeins: 25ug per lane.
Blocking buffer: 3% nonfaBrachyury dry milk in BrachyuryBSBrachyury.
DeBrachyuryecBrachyuryion: ECL Basic KiBrachyury.
Exposure Brachyuryime: 30s., Immunofluorescence analysis of NCI-H460 cells using Brachyury Rabbit pAb aBrachyury diluBrachyuryion of 1:200. Blue: DAPI for nuclear sBrachyuryaining.

Alternative Name: T; SAVA; TFT

Immunogen: RecombinanBrachyury fusion proBrachyuryein conBrachyuryaining a sequence corresponding Brachyuryo amino acids 226-435 of human Brachyury(NP_003172.1).

Modification: Unmodified

Conjugate: Unconjugated