BRD2 Polyclonal Antibody-BS9958M

BRD2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9958M-50, BS9958M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P25440

Host: Rabbit

Reactivity: Human

Applications: WB, IHC ICC FC

All Applications: WB: 1:500-1:2000 IHC: 1:50-1:200
ICC: 1:50-1:200 FC: 1:50-1:100

Background: The bromodomain-containing proteins include BRD2, BRD3, BRD4 and BRDT. BRD2 (RING3 protein) is a mitogen-activated nuclear protein whose gene is located in the human MHC II region, suggesting its relation to HLA-associated diseases. The gene encoding BRD3 (RING3-like protein) contains two bromodomains and maps to chromosome 9q34. BRD4 (HUNK1 protein) is a nuclear protein involved in the regulation of chromosomal dynamics during mitosis. The testis-specific bromodomain protein BRDT contains a PEST sequence, indicating that it undergoes rapid intracellular degradation. The bromodomain-containing proteins are ubiquitously expressed.

Purification and Purity: Protein A affinity purified.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of BRD2 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 88 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1:2000 dilution., Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BRD2 antibody. Counter stained with hematoxylin., Flow cytometric analysis of 293T cells with BRD2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Alternative Name: Bromodomain-containing protein 2; O27.1.1; Really interesting new gene 3 protein; BRD2; KIAA9001; RING3

Immunogen: Recombinant protein within human BRD2 aa 1-200.

Conjugate: Unconjugated

Modification: Unmodified