Product Description

BRD4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS72923-50, BS72923-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60885

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF

All Applications: WB 1:500 - 1:2000
IF 1:50 - 1:200

Background: The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: BRD4 polyclonal antibody detects endogenous levels of BRD4 protein.

Bioworld Molecular Weight: 200kDa

Immunogen: Recombinant protein of human BRD4

Conjugate: Unconjugated

Modification: Unmodified

Pathway: Examples of Crosstalk Between Post-translational Modifications

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using BRD4 antibody.