Product Description

BRG1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66590-50, MB66590-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: ATP-dependent chromatin remodeling complexes play an essential role in the regμlation of various nuclear processes, such as gene expression, DNA replication, and repair. The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecμle of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regμlatory elements within chromatin. The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regμlate gene activation, cell growth, the cell cycle, and differentiation processes. BRM and BRG1 are also considered to be tumor suppressors and their expression levels are severely reduced in several cancer cell lines.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Mouse

Applications: WB, IF/ICC, IP

Alternate Names: BAF190A, BRG1, SNF2B, SNF2L4, Transcription activator BRG1, ATP-dependent helicase SMARCA4, BRG1-associated factor 190A, BAF190A, Mitotic growth and transcription activator, Protein BRG-1, Protein brahma homolog 1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regμlator of chromatin subfamily A member 4

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human BRG1 protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of BRG1 protein.

Molecular Weight: ~ 220 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P51532

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only