Product Description

BRG1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90148-50, BS90148-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51532(Human) Q3TKT4(Mouse) Q8K1P7(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: BRG1 polyclonal antibody detects endogenous levels of BRG1 protein.

Bioworld Molecular Weight: 185 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: HepG2, ICC staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: BAF190A, SMARCA4, ATP dependent helicase SMARCA4, ATP-dependent helicase SMARCA4, BAF 190, BAF190, BAF190A, Brahma protein like 1, BRG1, BRG1 associated factor 190A, BRG1 protein, BRG1-associated factor 190A, BRM/SWI2 related gene 1, Global transcription activator homologous sequence, global transcription activator snf2l4, Homeotic gene regulator, hSNF2b, Mitotic growth and transcription activator, MRD16, Nuclear protein GRB1, Protein brahma homolog 1, Protein BRG-1, Protein BRG1, RTPS2, SMARC A4, SMARCA4, SMCA4, SNF2, SNF2 beta, SNF2 like 4, SNF2-beta, SNF2B, SNF2L4, SNF2LB, Sucrose nonfermenting like 4, SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4, SWI2, Transcription activator BRG1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified